Equipment is available for the application of alternating magnetic fields to cell cultures, tissues and live animals and for conducting magnetic and optical hyperthermia experiments.

AC magnetic field applicators for magnetic hyperthermia:
Two field applicators with interchangeable coils and variable frequencies, (H = 0-36 kA/m, f =200-828 kHz) 2.
2. Two induction heating units with customized coils (Ambrell EASYHEAT 8130LI 10 kW and EASYHEAT 5060 6 kW units) (H = 0-50 kA/m, f =181-444 kHz).
3. One 6 kW PowerCube64 HiPe induction heating unit (H = 12 kA/m, f = 890 kHz).
Workspace configurations are tailored for application to cell cultures, magnetic colloids and polymeric materials, including an in situ drug release measurement system using spectrophotometry.

Contact: Gerardo Goya (goya@unizar.es)

We have a non-commercial magnetic hyperthermia equipment, built in-house, specially adapted for the application of alternating magnetic fields to cell cultures at controlled temperature. The equipment generates a uniform magnetic field in an area of 25×25 mm in which a thermostatized chamber is placed where the sample is placed with several wells (e.g. 4) containing different cell cultures. The equipment allows simultaneous comparative studies of the effects of intracellular hyperthermia on control samples, and on different cell culture samples under the same magnetic field conditions and environment. The magnetic field parameters can be modulated between 5 mT and 120 mT and between 20 kHz and 200 kHz.

Location: Campus Río Ebro
Contact: Angel Millán (amillan@unizar.es, 876553347).

This magnetic hyperthermia equipment is commercial (D5 series, nanoScale Biomagnetics).
This equipment has three different possible coils:

– CAL: which has a single solenoid tube geometry and has an open glass dewar flask providing excellent thermal insulation with a turbomolecular or rotary vacuum pump. With it, 15 different frequencies can be selected from 146-772 kHz and the field amplitude ranges from 48-564 G depending on the frequency.
– Model G: which has a simple solenoid tube geometry but with a larger diameter than the previous one. Ten frequencies can be selected between 117-617 kHz.
– PC90 Coilset: Provides high intensity over a wide area near the top surface and attenuates rapidly on the vertical axis. Available in 90 mm diameter. Designed to work with the optional CAT accessory to control atmosphere and temperature in cell culture experiments. Choice of 7 different frequencies between 147-338 kHz.

Location: Laboratorio Bionanosurf, Edificio I+D, Campus Rio Ebro
Contact: Jesús Martínez de la Fuente (jmfuente@unizar.es), Ilaria Armenia (ilariarmenia@unizar.es)

This magnetic hyperthermia equipment is commercial (DM100-DM3, nanoScale Biomagnetics).
This applicator can be used for in vivo magnetic hyperthermia testing and also in vitro testing on three-dimensional models, such as collagen-based 3D gels. The device consists of:
1) A controller unit where experiments are designed and data are recorded.
2) An application unit where the animal (ideally small animals such as mice) is placed. It has a fiber optic temperature probe that can be placed in the anus of the animal to check its body temperature during treatment.
This device can generate alternating magnetic fields (AMF) at different field amplitudes (up to 31.8 kA/m) and various frequencies (71, 87, 105.5, 110, 124.5, 196, 228 and 377.5 kHz).
The device is located inside the animal house of the Centro de Investigaciones Biomédicas de Aragón (CIBA), so it allows experiments with the application of several MFA cycles since the animals do not have to leave the animal house. It also has a hot water pump with a kind of bed to keep the animals lying on a warm surface during the application of MFA and prevent them from suffering hypothermia. The animal house has a portable anesthesia device, which can be used and allows the animals to be anesthetized with isoflurane during the treatment.

Location: Animalario CIBA
Contact: Jesús Martínez de la Fuente (jmfuente@unizar.es), Laura Asín (lasin@unizar.es)

2W, 808 nm wavelength laser diode (MDL-III-808-2W, Changchun New Industries Optoelectronics Technology Co.
Optoelectronics Technology Co., China) equipped with an optical fiber and a fixed collimator ((f= 11.07 mm, NA= 0.26;Thorlabs, Germany). Photodiode sensor (PD300-3W, Ophir Laser Measurement Group, Israel) for calibration.

Location: CIBA
Contact: Manuel Arruebo (arruebom@unizar.es, 876554343)

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